Composite

Part:BBa_K1969007:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-08)


Nano-lantern(cAMP-1.6) regulated by ADH1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI.rc site found at 1379
    Illegal BsaI.rc site found at 1963
    Illegal SapI.rc site found at 2164


Design Notes

The expression of Nano-lantern(cAMP-1.6) may affect the sensing resolution of intracellular cAMP level, so creating expression cassettes with different expression level is critical for the project. This cassette is one of high expression level.


Source

Use a constitutive promoter ADH1 to mediate the expression of reporter protein Nano-lantern(cAMP-1.6).

References